Genetic recombination in Bacillus subtilis 168:: Contribution of Holliday junction processing functions in chromosome segregation

被引:49
作者
Carrasco, B
Cozar, MC
Lurz, R
Alonso, JC
Ayora, S
机构
[1] Univ Autonoma Madrid, Ctr Nacl Biotecnol, Dept Biotecnol Microbiana, CSIC, E-28049 Madrid, Spain
[2] Univ Autonoma Madrid, Dept Biol Mol, E-28049 Madrid, Spain
[3] Max Planck Inst Mol Genet, Berlin, Germany
关键词
D O I
10.1128/JB.186.17.5557-5566.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacillus subtilis mutants classified within the E (ruvA, DeltaruvB, DeltarecU, and recD) and eta (DeltarecG) epistatic groups, in an otherwise rec(+) background, render cells impaired in chromosomal segregation. A less-pronounced segregation defect in DeltarecA and Deltasms (DeltaradA) cells was observed. The repair deficiency of addAB, DeltarecO, DeltarecR, recH, DeltarecS, and DeltasubA cells did not correlate with a chromosomal segregation defect. The sensitivity of epsilon epistatic group mutants to DNA-damaging agents correlates with ongoing DNA replication at the time of exposure to the agents. The Asms (DeltaradA) and DeltasubA mutations partially suppress the DNA repair defect in ruvA and recD cells and the segregation defect in ruvA and DeltarecG cells. The Deltasms (Deltarad4) and DeltasubA mutations partially suppress the DNA repair defect of DeltarecU cells but do not suppress the segregation defect in these cells. The DeltarecA mutation suppresses the segregation defect but does not suppress the DNA repair defect in ArecU cells. These results result suggest that (i) the RuvAB and RecG branch migrating DNA helicases, the RecU Holliday junction (HJ) resolvase, and RecD bias HJ resolution towards noncrossovers and that (ii) Sms (RadA) and SubA proteins might play a role in the stabilization and or processing of HJ intermediates.
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页码:5557 / 5566
页数:10
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