Aptamer-based sensor arrays for the detection and quantitation of proteins

被引：260

作者：

Kirby, R

Cho, EJ

Gehrke, B

Bayer, T

Park, YS

Neikirk, DP

McDevitt, JT

Ellington, AD ^{[1
]}

机构：

[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA

[2] Univ Texas, Dept Elect & Comp Engn, Austin, TX 78712 USA

[3] Univ Texas, Inst Cell & Mol Biol, Austin, TX 78712 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 14期

关键词：

D O I：

10.1021/ac049858n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aptamer biosensors have been immobilized on beads, introduced into micromachined chips on the electronic tongue sensor array, and used for the detection and quantitation of proteins. Aptamer chips could detect proteins in both capture and sandwich assay formats. Unlike most protein-based arrays, the aptamer chips could be stripped and reused multiple times. The aptamer chips proved to be useful for screening aptamers from in vitro selection experiments and for sensitively quantitating the biothreat agent ricin.

引用

收藏

页码：4066 / 4075

页数：10

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