Stereochemical control in diastereoselective reduction of α-substituted-β-ketoesters using a reductase purified from Kluyveromyces marxianus

被引：11

作者：

Cha, JH

Pae, AN

Choi, KI

Cho, YS

Kim, WH

Han, YS

Yun, HC

Lee, JS

Koh, HY

Lee, L

机构：

[1] Korea Inst Sci & Technol, Div Life Sci, Seoul 130650, South Korea

[2] Daewoong Pharmaceut Co Ltd, Inst Biosci & Biotechnol, Yongin 449814, Kyunggi Do, South Korea

[3] Seoul Natl Univ, Sch Chem & Mol Engn, Seoul 151742, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 20期

关键词：

asymmetric reduction; biocatalyst; carbonyl reductase; diastereoselectivity; Kluyveromyces marxianus;

D O I：

10.1023/A:1020649316546

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An NADPH-dependent carbonyl reductase that shows (3R)-selective reducing activity for alpha-substituted-beta-ketoesters was purified from Kluyveromyces marxianus and racemic alkyl 2-substituted-3-oxobutanoates were reduced to the corresponding (2S,3R)- or (2R,3R)-2-substituted-3-hydroxybutanoates with enantiomeric purity (>99%) and diastereoselectivity (24 similar to 98%).

引用

收藏

页码：1695 / 1698

页数：4

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