Efficient Catalytic Promiscuity for Chemically Distinct Reactions

被引：43

作者：

Babtie, Ann C. ^{[1
]}

Bandyopadhyay, Subhajit ^{[1
]}

Olguin, Luis F. ^{[1
]}

Hollfelder, Florian ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 20期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

catalytic promiscuity; enzyme catalysis; hydrolases; phosphatases; sulfatases; COLI ALKALINE-PHOSPHATASE; PSEUDOMONAS-AERUGINOSA; TRANSITION-STATE; PHOSPHODIESTERASE ACTIVITY; ENZYME PROMISCUITY; SULFATASE ACTIVITY; HYDROLYSIS; EVOLUTION; ARYLSULFATASE; REACTIVITY;

D O I：

10.1002/anie.200805843

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

High catalytic proficiencies observed for the native and promiscuous reaction of the Pseudomonas aeruginosa arylsulfatase (PAS; the picture shows transition states of the two substrates with corresponding binding constants Ktx) suggest that the trade-off between high activity and tight specificity can be substantially relaxed. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3692 / 3694

页数：3

共 32 条

[1] The 'evolvability' of promiscuous protein functions
Aharoni, A
Gaidukov, L
Khersonsky, O
Gould, SM
Roodveldt, C
Tawfik, DS
[J]. NATURE GENETICS, 2005, 37 (01) : 73 - 76
[2] BEIL S, 1995, EUR J BIOCHEM, V229, P385, DOI 10.1111/j.1432-1033.1995.tb20479.x
[3] STUDIES ON SULFATE ESTERS .I. NUCLEOPHILIC REACTIONS OF AMINES WITH P-NITROPHENYL SULFATE
BENKOVIC, SJ
BENKOVIC, PA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (23) : 5504 - &
[4] Ohno's Model Revisited: Measuring the Frequency of Potentially Adaptive Mutations under Various Mutational Drifts
Bershtein, Shimon
Tawfik, Dan S.
[J]. MOLECULAR BIOLOGY AND EVOLUTION, 2008, 25 (11) : 2311 - 2318
[5] 1.3 Å structure of arylsulfatase from Pseudomonas aeruginosa establishes the catalytic mechanism of sulfate ester cleavage in the sulfatase family
Boltes, I
Czapinska, H
Kahnert, A
von Bülow, R
Dierks, T
Schmidt, B
von Figura, K
Kertesz, MA
Usón, I
[J]. STRUCTURE, 2001, 9 (06) : 483 - 491
[6] Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways
Bornscheuer, UT
Kazlauskas, RJ
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (45) : 6032 - 6040
[7] Mechanistic studies of β-arylsulfotransferase IV
Chapman, E
Bryan, MC
Wong, CH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) : 910 - 915
[8] CATALYTIC HYDROLYSIS OF CAMP
CHIN, J
ZOU, X
[J]. CANADIAN JOURNAL OF CHEMISTRY, 1987, 65 (08) : 1882 - 1884
[9] Enzymatic mechanisms of phosphate and sulfate transfer
Cleland, W. Wallace
Hengge, Alvan C.
[J]. CHEMICAL REVIEWS, 2006, 106 (08) : 3252 - 3278
[10] Posttranslational formation of formylglycine in prokaryotic sulfatases by modification of either cysteine or serine
Dierks, T
Miech, C
Hummerjohann, J
Schmidt, B
Kertesz, MA
von Figura, K
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (40) : 25560 - 25564

← 1 2 3 4 →