Enhanced simultaneous production of mycelia and intracellular polysaccharide in submerged cultivation of Cordyceps jiangxiensis using desirability functions

被引:74
作者
Xiao, Jian-hui [1 ]
Chen, Dai-xiong [1 ]
Wan, Wei-hong [1 ]
Hu, Xi-jie [1 ]
Qi, Ying [1 ]
Liang, Zong-qi [1 ]
机构
[1] Zunyi Med Coll, Affiliated Hosp, Key Lab Cell Engn Guizhou Province, Zunyi 563003, Peoples R China
关键词
Cordyceps jiangxiensis; desirability functions; intracellular polysaccharide; submerged cultivation; optimization;
D O I
10.1016/j.procbio.2006.03.031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of medium composition and fermentation parameters on simultaneous production of mycelia and intracellular polysaccharide by medicinal mushroom, Cordyceps jangxiensis were investigated in shake-flask Cultures using desirability functions. The optimal conditions were obtained via the associated desirability as follows: 40 g/l of molasses glycerol. 5 g/l of peanut steep meal. 6 g/l of yeast extract. 1 g/l of KH2PO4, 0.5 g/l of ZnCl2, 0.5 g/l of MgSO4, initial pH at 6, cultivation temperature at 28 degrees C, 200 nil of medium working volume in a 500 ml flask and 5% (v/ v) of inoculum size. Under these conditions, the production titer of mycelia and intracellular polysaccharide reached 24.5 and 8.9 g/l. respectively, after a 9-day submerged cultivation. The use of desirability functions was proved effective in optimizing multi-response fermentation processes and it is readily applicable to other fermentation systems. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1887 / 1893
页数:7
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