The discriminatory power of ribo-PCR compared to conventional ribotyping for epidemiological purposes

被引：6

作者：

Severino, P

Darini, AL

Magalhaes, VD

机构：

[1] Hosp Israelita Albert Einstein, Mol Biol Lab, BR-05651901 Sao Paulo, Brazil

[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Clin Anal Dept, BR-14049 Ribeirao Preto, SP, Brazil

来源：

APMIS | 1999年 / 107卷 / 12期

关键词：

ribotyping; ribo-PCR; epidemiological relationships;

D O I：

10.1111/j.1699-0463.1999.tb01511.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Molecular typing techniques have become increasingly important for confirmation of epidemiological relationships and delimitation of nosocomial outbreaks. The discriminatory power of the two DNA-based typing methods, conventional ribotyping and ribo-PCR, was assessed to distinguish between selected strains of Acinetobacter calcoaceticus, Enterobacter cloacae, Serratia marcescens and Pseudomonas aeruginosa. Overall, conventional ribotyping was more discriminatory than ribo-PCR.

引用

收藏

页码：1079 / 1084

页数：6

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