Generation of whole-chromosome painting probes specific to each chicken macrochromosome

被引:11
作者
Guillier-Gencik, Z [1 ]
Bernheim, A [1 ]
Coullin, P [1 ]
机构
[1] Inst Gustave Roussy, UMR 1599, Lab Cytogenet & Genet Oncol, F-94805 Villejuif, France
来源
CYTOGENETICS AND CELL GENETICS | 1999年 / 87卷 / 3-4期
关键词
D O I
10.1159/000015450
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The realization of physical and genetic maps of the chicken genome is dependent on progress in cytogenetic knowledge of its karyotype, To help achieve this goal, we constructed amplified representative DNA samples of the chicken chromosomes 1, 2, 3, 4, 5, 6, 7, 8, Z, W and of the terminal heterochromatin part of Zq by chromosome microdissection and DOP-PCR amplification. These chromosome DNA samples, which represent about 75 % of the chicken genome, were used to generate whole chromosome painting probes for FISH, The direct application of these chromosome specific probes is dual FISH localization and characterization of panels of chicken interspecific somatic hybrids. We discuss some aspects of the chicken genome and its repeated sequences, Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:282 / 285
页数:4
相关论文
共 19 条
[1]   Development of a chicken Z-chromosome-specific DNA library [J].
Ambady, S ;
Ciufo, S ;
Robl, JM ;
Smyth, JR ;
deLeon, FAP .
JOURNAL OF HEREDITY, 1997, 88 (03) :247-249
[2]   REVERSE CHROMOSOME PAINTING - A METHOD FOR THE RAPID ANALYSIS OF ABERRANT CHROMOSOMES IN CLINICAL CYTOGENETICS [J].
CARTER, NP ;
FERGUSONSMITH, MA ;
PERRYMAN, MT ;
TELENIUS, H ;
PELMEAR, AH ;
LEVERSHA, MA ;
GLANCY, MT ;
WOOD, SL ;
COOK, K ;
DYSON, HM ;
FERGUSONSMITH, ME ;
WILLATT, LR .
JOURNAL OF MEDICAL GENETICS, 1992, 29 (05) :299-307
[3]  
Chudoba I, 1996, ONCOL REP, V3, P829
[4]   Preliminary linkage map of the chicken (Gallus domesticus) genome based on microsatellite markers: 77 new markers mapped [J].
Crooijmans, RPMA ;
vanOers, PAM ;
Strijk, JA ;
vanderPoel, JJ ;
Groenen, MAM .
POULTRY SCIENCE, 1996, 75 (06) :746-754
[5]   Efficient modification of human chromosomal alleles using recombination-proficient chicken human microcell hybrids [J].
Dieken, ES ;
Epner, EM ;
Fiering, S ;
Fournier, REK ;
Groudine, M .
NATURE GENETICS, 1996, 12 (02) :174-182
[6]   DNA-SEQUENCE ORGANIZATION IN AVIAN GENOMES [J].
EPPLEN, JT ;
ENGEL, W ;
LEIPOLDT, M ;
SCHMIDTKE, J .
CHROMOSOMA, 1978, 69 (03) :307-321
[7]   Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH) [J].
Fillon, V ;
Morisson, M ;
Zoorob, R ;
Auffray, C ;
Douaire, M ;
Gellin, J ;
Vignal, A .
CHROMOSOME RESEARCH, 1998, 6 (04) :307-313
[8]   GENERATION OF BAND-SPECIFIC PAINTING PROBES FROM A SINGLE MICRODISSECTED CHROMOSOME [J].
GUAN, XY ;
TRENT, JM ;
MELTZER, PS .
HUMAN MOLECULAR GENETICS, 1993, 2 (08) :1117-1121
[9]   Characterization of DNA sequences constituting the terminal heterochromatin of the chicken Z chromosome [J].
Hori, T ;
Suzuki, Y ;
Solovei, I ;
Saitoh, Y ;
Hutchison, N ;
Ikeda, JE ;
Macgregor, H ;
Mizuno, S .
CHROMOSOME RESEARCH, 1996, 4 (06) :411-426
[10]  
LUEDECKE H-J, 1989, Nature (London), V338, P348