Generation of whole-chromosome painting probes specific to each chicken macrochromosome

被引：11

作者：

Guillier-Gencik, Z ^{[1
]}

Bernheim, A ^{[1
]}

Coullin, P ^{[1
]}

机构：

[1] Inst Gustave Roussy, UMR 1599, Lab Cytogenet & Genet Oncol, F-94805 Villejuif, France

来源：

CYTOGENETICS AND CELL GENETICS | 1999年 / 87卷 / 3-4期

关键词：

D O I：

10.1159/000015450

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The realization of physical and genetic maps of the chicken genome is dependent on progress in cytogenetic knowledge of its karyotype, To help achieve this goal, we constructed amplified representative DNA samples of the chicken chromosomes 1, 2, 3, 4, 5, 6, 7, 8, Z, W and of the terminal heterochromatin part of Zq by chromosome microdissection and DOP-PCR amplification. These chromosome DNA samples, which represent about 75 % of the chicken genome, were used to generate whole chromosome painting probes for FISH, The direct application of these chromosome specific probes is dual FISH localization and characterization of panels of chicken interspecific somatic hybrids. We discuss some aspects of the chicken genome and its repeated sequences, Copyright (C) 2000 S. Karger AG, Basel.

引用

页码：282 / 285

页数：4

共 19 条

[11] The ZW lampbrush chromosomes of birds: a unique opportunity to look at the molecular cytogenetics of sex chromosomes [J].

Mizuno, S ;

Macgregor, H .

CYTOGENETICS AND CELL GENETICS, 1998, 80 (1-4) :149-157

[12] LOCALIZATION OF THE TELOMERIC (TTAGGG)N SEQUENCE IN CHICKEN (GALLUS-DOMESTICUS) CHROMOSOMES [J].

NANDA, I ;

SCHMID, M .

CYTOGENETICS AND CELL GENETICS, 1994, 65 (03) :190-193

[13] GENE-MAPPING IN CHICKEN-CHINESE HAMSTER SOMATIC-CELL HYBRIDS - SERUM-ALBUMIN AND PHOSPHOGLUCOMUTASE-2 STRUCTURAL GENES ON CHICKEN CHROMOSOME-6 [J].

PALMER, DK ;

JONES, C .

JOURNAL OF HEREDITY, 1986, 77 (02) :106-108

[14] Chromosome microdissection and microcloning [J].

Saitoh, Y ;

Ikeda, JE .

CHROMOSOME RESEARCH, 1997, 5 (02) :77-80

[15] Gene mapping by chromosome microdissection and microisolation in the chicken [J].

Shaw, EM ;

Otis, JS ;

Lamont, SJ ;

Guise, KS ;

Shoffner, RN .

POULTRY SCIENCE, 1996, 75 (01) :6-12

[16] Comparative painting reveals strong chromosome homology over 80 million years of bird evolution [J].

Shetty, S ;

Griffin, DK ;

Graves, JAM .

CHROMOSOME RESEARCH, 1999, 7 (04) :289-295

[17]

Solovei I, 1999, CYTOGENET CELL GENET, V85, P19

[18] CYTOGENETIC ANALYSIS BY CHROMOSOME PAINTING USING DOP-PCR AMPLIFIED FLOW-SORTED CHROMOSOMES [J].

TELENIUS, H ;

PELMEAR, AH ;

TUNNACLIFFE, A ;

CARTER, NP ;

BEHMEL, A ;

FERGUSONSMITH, MA ;

NORDENSKJOLD, M ;

PFRAGNER, R ;

PONDER, BAJ .

GENES CHROMOSOMES & CANCER, 1992, 4 (03) :257-263

[19] Generation of chicken Z-chromosome painting probes by microdissection for screening large-insert genomic libraries [J].

Zimmer, R ;

King, WA ;

Gibbins, AMV .

CYTOGENETICS AND CELL GENETICS, 1997, 78 (02) :124-130

← 1 2 →