Microdissection and FISH investigations in acute myeloid leukemia: A step forward to full identification of complex karyotypic changes

被引：8

作者：

Falzetti, D

Vermeesch, JR

Matteucci, C

Ciolli, S

Martelli, MF

Marynen, P

Mecucci, C

机构：

[1] Univ Perugia, Dept Hematol, I-06100 Perugia, Italy

[2] Katholieke Univ Leuven, Ctr Human Genet, Louvain, Belgium

[3] Katholieke Univ Leuven, Interuniv Inst Biotechnol, Louvain, Belgium

[4] Div Hematol, Florence, Italy

来源：

CANCER GENETICS AND CYTOGENETICS | 2000年 / 118卷 / 01期

关键词：

D O I：

10.1016/S0165-4608(99)00189-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Complex chromosomal rearrangements in malignant hemopathies frequently remain unclarified because of paucity of material for further fluorescence in situ hybridization analyses and/or lack of suitable probes. Chromosome microdissection (MD) can be an adequate approach to elucidate chromosome aberrations unrecognizable by conventional karyotyping. We applied MD in two patients with acute myeloid leukemia (AML) and unidentified chromosome changes at karyotype, Microdissection of a ring chromosome in an AML-M5 case revealed 21q polysomy. In an AML-M4 case, MD of on add(15p) disclosed a t(8;15) with over-representotion of both 8q22 and 8q24 bands. YAC probes were helpful in showing duplication of the ETO gene at 8q22, and amplification of C-MYC, at 8q24. (C) Elsevier Science Inc., 2000, All rights reserved.

引用

页码：28 / 34

页数：7

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