An extra dimension in nucleic acid sequence recognition

被引：39

作者：

Fox, Keith R.

Brown, Tom

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Sch Biol Sci, Southampton SO17 1BJ, Hants, England

来源：

QUARTERLY REVIEWS OF BIOPHYSICS | 2005年 / 38卷 / 04期

关键词：

D O I：

10.1017/S0033583506004197

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored.

引用

收藏

页码：311 / 320

页数：10

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