The Wnt Receptor, Lrp5, Is Expressed by Mouse Mammary Stem Cells and Is Required to Maintain the Basal Lineage

被引:104
作者
Badders, Nisha M. [1 ]
Goel, Shruti [1 ]
Clark, Rod J. [1 ]
Klos, Kristine S. [1 ]
Kim, Soyoung [1 ]
Bafico, Anna [2 ]
Lindvall, Charlotta [3 ]
Williams, Bart O. [3 ]
Alexander, Caroline M. [1 ]
机构
[1] Univ Wisconsin, McArdle Lab Canc Res, Madison, WI 53706 USA
[2] Cardiff Univ, Dept Pathol, Cardiff, S Glam, Wales
[3] Van Andel Res Inst, Lab Cell Signaling & Carcinogenesis, Grand Rapids, MI USA
来源
PLOS ONE | 2009年 / 4卷 / 08期
关键词
GENE-EXPRESSION; GLAND DEVELOPMENT; SIGNALING PATHWAYS; CANCER; P63; INTERNALIZATION; PROLIFERATION; ACCUMULATION; PROTEIN; DIFFERENTIATION;
D O I
10.1371/journal.pone.0006594
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Ectopic Wnt signaling induces increased stem/ progenitor cell activity in the mouse mammary gland, followed by tumor development. The Wnt signaling receptors, Lrp5/6, are uniquely required for canonical Wnt activity. Previous data has shown that the absence of Lrp5 confers resistance to Wnt1-induced tumor development. Methodology/Principal Findings: Here, we show that all basal mammary cells express Lrp5, and co-express Lrp6 in a similar fashion. Though Wnt dependent transcription of key target genes is relatively unchanged in mammary epithelial cell cultures, the absence of Lrp5 specifically depletes adult regenerative stem cell activity (to less than 1%). Stem cell activity can be enriched by >200 fold (over 80% of activity), based on high Lrp5 expression alone. Though Lrp5 null glands have apparent normal function, the basal lineage is relatively reduced (from 42% basal/total epithelial cells to 22%) and Lrp5(-/-) mammary epithelial cells show enhanced expression of senescence-associated markers in vitro, as measured by expression of p16(Ink4a) and TA-p63. Conclusions/Significance: This is the first single biomarker that has been demonstrated to be functionally involved in stem cell maintenance. Together, these results demonstrate that Wnt signaling through Lrp5 is an important component of normal mammary stem cell function.
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页数:14
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