Monoclonal antibody CD133-2 (AC141) against hematopoietic stem cell antigen CD133 shows crossreactivity with cytokeratin 18

被引:28
作者
Pötgens, AJG [1 ]
Schmitz, U [1 ]
Kaufmann, P [1 ]
Frank, HG [1 ]
机构
[1] Univ Hosp Aachen, Dept Anat, D-52057 Aachen, Germany
关键词
CD133; AC141; PROML1; cytokeratin; 18; hematopoietic stem cells; epithelial cells; human;
D O I
10.1177/002215540205000814
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CD133 is an antigen expressed on hematopoietic progenitor cells and on some epithelial cells. We previously reported that a commercially available antibody against CD133, CD133-2/AC141, also reacted with an intracellular protein in placental trophoblasts. Here we show by 2D electrophoresis and mass spectroscopy that this reactivity is with cytokeratin 18, a cytokeratin present in most simple epithelia. Immunohistochemistry (IHC) with CD133-2/AC141 on a trophoblast cell line displayed a staining pattern typical for the cytoskeleton. Cryostat sections of stratified epithelia lacking cytokeratin 18 did not react with CD133-2/AC141. In conclusion, care must betaken not to misinterpret staining patterns using CD133-2/AC141 in IHC.
引用
收藏
页码:1131 / 1134
页数:4
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