Synthesis of Semitelechelic Maleimide Poly(PEGA) for Protein Conjugation By RAFT Polymerization

被引：88

作者：

Bays, Emmanuelle

Tao, Lei

Chang, Chien-Wen

Maynard, Heather D. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

BIOMACROMOLECULES | 2009年 / 10卷 / 07期

基金：

美国国家科学基金会;

关键词：

LIVING RADICAL POLYMERIZATION; FRAGMENTATION CHAIN TRANSFER; LABELED SIDE-CHAINS; PEGYLATION; POLYMERS; CHEMISTRY; CYSTEINE; DESIGN; THERAPEUTICS; STABILITY;

D O I：

10.1021/bm9001987

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Maleimide end functionalized polymers for site-selective conjugation to free cysteines of proteins were synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization. A furan-protected maleimide chain transfer agent (CTA) was employed in the RAFT polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA). Gel permeation chromatography (GPC) with laser light scattering detection indicated that 20000 and 39000 Da polyPEGA had been made with polydispersity indices of 1.25 and 1.36, respectively. The maleimide group on the polymer chain end was exposed by heating the poly(PEGA)s for 4 h. The deprotection efficiency was estimated to be 80 and 60% for poly(PEGA)(20) (kDa) and poly(PEGA)(39) (kDa), respectively. Maleimide-poly(PEGA)s were conjugated to V131C T4 lysozyme (T4L), and the resultant polymer-protein conjugates were characterized by size exclusion chromatography and gel electrophoresis.

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收藏

页码：1777 / 1781

页数：5

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