An unusual "senseless" 2′,5′-oligoribonucleotide with potent anti-HIV activity

被引：10

作者：

Aboul-Fadl, T ^{[1
]}

Agrawal, VK ^{[1
]}

Buckheit, RW ^{[1
]}

Broom, AD ^{[1
]}

机构：

[1] Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2004年 / 23卷 / 03期

关键词：

oligoribonucleotide; HIV-1; reverse transcriptase; ssRNA;

D O I：

10.1081/NCN-120030713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A new 32-mer 2',5'-oligoribonucleotide of 1-methyl-6-thioinosinic acid (10) has been synthesized. The design of this unique oligoribonucleotide is based on the reported HIV inhibitory activities of both 2',5'-oligonucleotides and the 3',5'-oligoribonucleotides containing the 1-methyl-6-mercaptopurine base. Tm and CD studies of 10 revealed that it has no organized secondary structure, presumably due to the rigidity of the molecule. The synthesized oligomer, 10, showed a potent inhibitory effect on HIV-1(RF) and significantly inhibited HIV-1 reverse transcriptase.

引用

页码：545 / 554

页数：10

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