Rapid isocratic HPLC analysis of hypericins

被引：29

作者：

Sirvent, T

Gibson, DM ^{[1
]}

机构：

[1] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14850 USA

[2] USDA ARS, US Plant Soil & Nutr Lab, Plant Protect Res Unit, Ithaca, NY 14853 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 02期

关键词：

D O I：

10.1081/JLC-100101449

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a new method for the analysis of hypericins from crude extracts of Hypericum perforatum (St. John's Wort) using HPLC. Hypericin and pseudohypericin and related compounds were separated in 15 minutes using a simple isocratic elution method on a modified phenyl column. This isocratic method permits the quantitative and rapid analysis of hypericins in crude acetone samples. In addition, we report on the photoconversion of protohypericins to hypericins, as well as the stability of hypericin and pseudohypericin as a function of light, time, and storage conditions.

引用

页码：251 / 259

页数：9

共 10 条

[1] Identification by high-performance liquid chromatography diode array detection mass spectrometry and quantification by high-performance liquid chromatography UV absorbance detection of active constituents of Hypericum perforatum [J].

Brolis, M ;

Gabetta, B ;

Fuzzati, N ;

Pace, R ;

Panzeri, F ;

Peterlongo, F .

JOURNAL OF CHROMATOGRAPHY A, 1998, 825 (01) :9-16

[2]

HABERLEIN H, 1992, PZ (Pharmazeutische Zeitung) Wissenschaft, V137, P169

[3]

HOLZL VJ, 1987, DTSCH APOTHEKER ZEIT, V23, P1227

[4]

Kurth H, 1998, ADV THER, V15, P117

[5] A METHOD FOR THE QUANTITATION OF HYPERICIN, AN ANTIVIRAL AGENT, IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

LIEBES, L ;

MAZUR, Y ;

FREEMAN, D ;

LAVIE, D ;

LAVIE, G ;

KUDLER, N ;

MENDOZA, S ;

LEVIN, B ;

HOCHSTER, H ;

MERUELO, D .

ANALYTICAL BIOCHEMISTRY, 1991, 195 (01) :77-85

[6] A review of 12 commonly used medicinal herbs [J].

O'Hara, M ;

Kiefer, D ;

Farrell, K ;

Kemper, K .

ARCHIVES OF FAMILY MEDICINE, 1998, 7 (06) :523-536

[7] Determination of naphthodianthrones in plant extracts from Hypericum perforatum L by liquid chromatography electrospray mass spectrometry [J].

Piperopoulos, G ;

Lotz, R ;

Wixforth, A ;

Schmierer, T ;

Zeller, KP .

JOURNAL OF CHROMATOGRAPHY B, 1997, 695 (02) :309-316

[8]

SCHUETT H, 1996, MORPHOLOGISCHE PHYTO

[9]

UPTON R, 1997, QUALITY CONTROL ANAL, P1

[10]

ZEVAKOVA VA, 1991, SOEDIN, P138

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