Cellular prion protein and caveolin-1 interaction in a neuronal cell line precedes Fyn/Erk 1/2 signal transduction

被引：43

作者：

Toni, Mattia

Spisni, Enzo

Griffoni, Cristiana

Santi, Spartaco

Riccio, Massimo

Lenaz, Patrizia

Tomasi, Vittorio

机构：

[1] Univ Bologna, Dept Expt Biol, I-40126 Bologna, Italy

[2] CNR, Inst Cytomorphol, I-40136 Bologna, Italy

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2006年

关键词：

D O I：

10.1155/JBB/2006/69469

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

It has been reported that cellular prion protein (PrPc) is enriched in caveolae or caveolae-like domains with caveolin-1 (Cav-1) participating to signal transduction events by Fyn kinase recruitment. By using the Glutathione-S-transferase (GST)-fusion proteins assay, we observed that PrPc strongly interacts in vitro with Cav-1. Thus, we ascertained the PrPc caveolar localization in a hypothalamic neuronal cell line (GN11), by confocal microscopy analysis, flotation on density gradient, and coimmunoprecipitation experiments. Following the anti-PrPc antibody-mediated stimulation of live GN11 cells, we observed that PrPc clustered on plasma membrane domains rich in Cav-1 in which Fyn kinase converged to be activated. After these events, a signaling cascade through p42/44 MAP kinase (Erk 1/2) was triggered, suggesting that following translocations from rafts to caveolae or caveolae-like domains PrPc could interact with Cav-1 and induce signal transduction events.

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页数：13

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