Identification of a novel human eicosanoid receptor coupled to Gi/o

被引:137
作者
Hosoi, T [1 ]
Koguchi, Y [1 ]
Sugikawa, E [1 ]
Chikada, A [1 ]
Ogawa, K [1 ]
Tsuda, N [1 ]
Suto, N [1 ]
Tsunoda, S [1 ]
Taniguchi, T [1 ]
Ohnuki, T [1 ]
机构
[1] Tanabe Seiyaku Co Ltd, Discovery Res Lab, Toda, Saitama 3358505, Japan
关键词
D O I
10.1074/jbc.M203194200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have conducted an in silico data base search for and cloned a novel G-protein-coupled receptor (GPCR) named TG1019. Dot and Northern blotting analyses showed that transcripts of the novel GPCR were expressed in various tissues except brain, and the expression was more intense in liver, kidney, peripheral leukocyte, lung, and spleen than in other tissues. By GTPgammaS binding assay using the TG1019-Galpha(il)-protein fusion expressed in insect cells, eicosanoids, and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-oxo-ETE), 5(S)-hydroperoxy-6E,8Z, 11Z,14Z-eicosatetraenoic acid, and arachidonic acid were identified to exhibit agonistic activities against TG1019. 5-oxo-ETE was the most potent to enhance the specific binding by 6-fold at a maximum effect dose of submicromolar to micromolar order with an ED50 value of 5.7 nm. Conversely, polyunsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid showed antagonistic activities against TG1019. In Chinese hamster ovary cells transiently expressing TG1019, the forskolin-stimulated production of cAMP was inhibited up to similar to70% by 5-oxo-ETE, with an IC50 value of 33 nm. This inhibition was sensitive to pretreatment of the cells with pertussis toxin.
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页码:31459 / 31465
页数:7
相关论文
共 45 条
[1]  
ABRAMOVITZ M, 1994, J BIOL CHEM, V269, P2632
[2]   CLONING AND CHARACTERIZATION OF CDNA-ENCODING HUMAN A-TYPE ENDOTHELIN RECEPTOR [J].
ADACHI, M ;
YANG, YY ;
FURUICHI, Y ;
MIYAMOTO, C .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (03) :1265-1272
[3]   Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells [J].
Akbar, GKM ;
Dasari, VR ;
Webb, TE ;
Ayyanathan, K ;
Pillarisetti, K ;
Sandhu, AK ;
Athwal, RS ;
Daniel, JL ;
Ashby, B ;
Barnard, EA ;
Kunapuli, SP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (31) :18363-18367
[4]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[5]   Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14 [J].
Ames, RS ;
Sarau, HM ;
Chambers, JK ;
Willette, RN ;
Alyar, NV ;
Romanic, AM ;
Louden, CS ;
Foley, JJ ;
Sauermelch, CF ;
Coatney, RW ;
Ao, ZH ;
Disa, J ;
Holmes, SD ;
Stadel, JM ;
Martin, JD ;
Liu, WS ;
Glover, GI ;
Wilson, S ;
McNulty, DE ;
Ellis, CE ;
Elshourbagy, NA ;
Shabon, U ;
Trill, JJ ;
Hay, DWP ;
Ohlstein, EH ;
Bergsma, DJ ;
Douglas, SA .
NATURE, 1999, 401 (6750) :282-286
[6]   Hydrophobicity of residue351 of the G protein Gi1α determines the extent of activation by the α2A-adrenoceptor [J].
Bahia, DS ;
Wise, A ;
Fanelli, F ;
Lee, M ;
Rees, S ;
Milligan, G .
BIOCHEMISTRY, 1998, 37 (33) :11555-11562
[7]  
BASTIEN L, 1994, J BIOL CHEM, V269, P11873
[8]   MOLECULAR-CLONING AND CHARACTERIZATION OF THE HUMAN PROSTANOID DP RECEPTOR [J].
BOIE, Y ;
SAWYER, N ;
SLIPETZ, DM ;
METTERS, KM ;
ABRAMOVITZ, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (32) :18910-18916
[9]  
BOIE Y, 1994, J BIOL CHEM, V269, P12173
[10]   A G protein-coupled receptor for UDP-glucose [J].
Chambers, JK ;
Macdonald, LE ;
Sarau, HM ;
Ames, RS ;
Freeman, K ;
Foley, JJ ;
Zhu, Y ;
McLaughlin, MM ;
Murdock, P ;
McMillan, L ;
Trill, J ;
Swift, A ;
Aiyar, N ;
Taylor, P ;
Vawter, L ;
Naheed, S ;
Szekeres, P ;
Hervieu, G ;
Scott, C ;
Watson, JM ;
Murphy, AJ ;
Duzic, E ;
Klein, C ;
Bergsma, DJ ;
Wilson, S ;
Livi, GP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (15) :10767-10771