CLONING AND CHARACTERIZATION OF CDNA-ENCODING HUMAN A-TYPE ENDOTHELIN RECEPTOR

被引：108

作者：

ADACHI, M ^{[1
]}

YANG, YY ^{[1
]}

FURUICHI, Y ^{[1
]}

MIYAMOTO, C ^{[1
]}

机构：

[1] NIPPON ROCHE RES CTR,DEPT MOLEC GENET,KAMAKURA 247,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)81332-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA coding for the human A-type endothelin receptor (ETA) was cloned from a human placenta cDNA library. The cDNA contained the entire coding sequence for the 427 amino acid protein with a relative Mr of 48,722. The deduced amino acid sequence of the human ETA was, respectively, 94% and 93% homologous with the sequence of bovine ETA and rat ETA, but was only 64% homologous with that of the human ETB receptor. Upon expression in COS-1 cells, the human ETA receptor showed binding activity to ETA, with the highest selectivity to ET-1. Northern blot analysis showed that the mRNA of human placenta ETA consists of one species 5 kilo-nucleotides in length, and the same analysis for the uterus, testis, heart and adrenal gland of Cynomolgus monkey showed that the cognate mRNAs are widely distributed. © 1991 Academic Press, Inc.

引用

页码：1265 / 1272

页数：8

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