Mismatch repair-driven mutational bias in D. melanogaster

被引：40

作者：

Harr, B ^{[1
]}

Todorova, J ^{[1
]}

Schlötterer, C ^{[1
]}

机构：

[1] Vet Med Univ Wien, Inst Tierzucht & Genet, A-1210 Vienna, Austria

来源：

MOLECULAR CELL | 2002年 / 10卷 / 01期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1016/S1097-2765(02)00575-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used microsatellite sequences to evaluate the influence of the mismatch repair system on mutation bias in D. melanogaster. While mismatch-proficient cells have the highest mutation rate at (GT)(n) repeats, (AT), repeats were the least stable ones in spe/1(-)/(-) flies lacking functional mismatch repair. Furthermore, the mutation spectrum of long microsatellite alleles in spel1(-)/(-) was slightly upward biased, resulting in a gain of repeats, whereas wild-type flies have a strong downward bias. Interestingly, this mismatch repair-mediated downward mutation bias is reflected in the genome composition of D. melanogaster. When compared to other species, D. melanogaster has significantly shorter microsatellites. Our results suggest that the mismatch repair system may have an important role in shaping genome composition.

引用

页码：199 / 205

页数：7

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