Rapid Molecular Genotyping and Clonal Complex Assignment of Staphylococcus aureus Isolates by PCR Coupled to Electrospray Ionization-Mass Spectrometry

被引：45

作者：

Hall, Thomas A. ^{[2
]}

Sampath, Rangarajan ^{[2
]}

Blyn, Lawrence B. ^{[2
]}

Ranken, Raymond ^{[2
]}

Ivy, Cristina ^{[2
]}

Melton, Rachael ^{[2
]}

Matthews, Heather ^{[2
]}

White, Neill ^{[2
]}

Li, Feng ^{[2
]}

Harpin, Vanessa ^{[2
]}

Ecker, David J. ^{[2
]}

McDougal, Linda K. ^{[3
]}

Limbago, Brandi ^{[3
]}

Ross, Tracy ^{[1
]}

Wolk, Donna M. ^{[4
]}

Wysocki, Vicki ^{[4
]}

Carroll, Karen C. ^{[1
]}

机构：

[1] Johns Hopkins Univ Hosp, Baltimore, MD 21287 USA

[2] Ibis Biosci Inc, Carlsbad, CA 92008 USA

[3] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA

[4] Univ Arizona, Tucson, AZ USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 06期

关键词：

FIELD GEL-ELECTROPHORESIS; SOFT-TISSUE INFECTIONS; METHICILLIN-RESISTANT; UNITED-STATES; UNIVERSAL BIOSENSOR; STRAIN; IDENTIFICATION; COMMUNITY; EPIDEMIOLOGY; SURVEILLANCE;

D O I：

10.1128/JCM.02175-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (MLST). The method was used to identify the clonal complex and USA type of each isolate and is suitable for use in a clinical or public-health setting. The method was validated using a panel of diverse isolates from the Centers for Disease Control and Prevention that were previously characterized by MLST and pulsed-field gel electrophoresis (PFGE). Clinical isolates from two geographically distinct hospitals were characterized, and the clustering results were in agreement with those for repetitive-element PCR and PFGE. The PCR/ESI-MS method enables genotyping of over 180 samples of S. aureus per day in an automated fashion.

引用

页码：1733 / 1741

页数：9

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