Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing

被引：85

作者：

Singh, DP

Kimura, A

Chylack, LT

Shinohara, T

机构：

[1] Brigham & Womens Hosp, Ctr Ophthalm Res, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA 02115 USA

来源：

GENE | 2000年 / 242卷 / 1-2期

关键词：

alternative splice; chromosome; 9p22.2; exon/intron; gene; LEDGF/p75 and p52;

D O I：

10.1016/S0378-1119(99)00506-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A human gene that encodes lens epithelium-derived growth factor (LEDGF) was isolated, and the DNA sequence and the exon/intron organization was determined. The gene contains at least IS exons and 14 introns and encodes LEDGF mRNA and p52 mRNA. Exons 1-15 encode LEDGF mRNA, and exons 1-9, and a part of the ninth intron encode a splice variant (p52). Sequences of the exon/intron junctions of the gene have the highly conserved GT/AG rule. Most intron/exon junctions correspond to junctions of individual protein motifs. Almost equal amounts of LEDGF and p52 are expressed in lens epithelial cells in culture. The LEDGF gene is assigned to chromosome 9p22.2, which is adjacent to the major cell malignancy locus. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：265 / 273

页数：9

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