Investigation of quinocetone-induced genotoxicity in HepG2 cells using the comet assay, cytokinesis-block micronucleus test and RAPD analysis

被引：54

作者：

Lin, Xi ^{[1
]}

Chen, Qian ^{[1
]}

Tang, Shu-Sheng ^{[1
]}

Zou, Jia-Jie ^{[1
]}

Chen, Kai-Pao ^{[1
]}

Zhang, Ting ^{[1
]}

Xiao, Xi-Long ^{[1
]}

机构：

[1] China Agr Univ, Dept Pharmacol & Toxicol, Coll Vet Med, Beijing 100193, Peoples R China

来源：

TOXICOLOGY IN VITRO | 2009年 / 23卷 / 07期

关键词：

Quinocetone; Genotoxicity; Comet assay; CBMN test; RAPD analysis; AMPLIFIED POLYMORPHIC DNA; HUMAN HEPATOMA-CELLS; MASS-SPECTROMETRY; OLAQUINDOX; CARBADOX; DAMAGE; METABOLITES; CARCINOGENESIS; IDENTIFICATION; MUTAGENESIS;

D O I：

10.1016/j.tiv.2009.07.038

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Quinocetone, a new quinoxaline 1,4-dioxide derivative, has been approved as an animal growth promoter in China since 2003. To investigate the genotoxicity of quinocetone in vitro its effects on the extent of, DNA injury in human hepatoma (HepG2) cells accompanied by chromosomal damage and genomic DNA alterations were tested. The cell viability test indicated that quinocetone inhibited cell proliferation as a function of dose and time. In the comet assay, significant DNA fragment migration was observed in a dose-dependent manner. A dose-dependent increase of the micronucleated (MN) cell frequency was shown in cytokinesis-block micronucleus (CBMN) test. The gain/loss of randomly amplified polymorphic DNA (RAPD) bands and the change of band intensity in RAPD profiles were obtained after HepG2 cells were exposed to quinocetone at concentrations of 1.25, 2.5 and 5 mu g/mL The results demonstrated that quinocetone exerted genotoxic effects on HepG2 cells. Thus, the use of quinocetone as a growth promoter in animal feed should be seriously considered. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1209 / 1214

页数：6

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