Cytokinesis-block micronucleus cytome assay

被引：1524

作者：

Fenech, Michael ^{[1
]}

机构：

[1] CSIRO Human Nutr, Genome Hlth Nutrigenom Lab, Adelaide, SA 5000, Australia

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 05期

关键词：

D O I：

10.1038/nprot.2007.77

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytokinesis-block micronucleus cytome assay is a comprehensive system for measuring DNA damage, cytostasis and cytotoxicity. DNA damage events are scored specifically in once-divided binucleated ( BN) cells and include ( a) micronuclei ( MNi), a biomarker of chromosome breakage and/ or whole chromosome loss, ( b) nucleoplasmic bridges ( NPBs), a biomarker of DNA misrepair and/ or telomere end-fusions, and ( c) nuclear buds ( NBUDs), a biomarker of elimination of amplified DNA and/ or DNA repair complexes. Cytostatic effects are measured via the proportion of mono-, bi- and multinucleated cells and cytotoxicity via necrotic and/ or apoptotic cell ratios. Further information regarding mechanisms leading to MNi, NPBs and NBUDs formation is obtained using centromere and/ or telomere probes. The assay is being applied successfully for biomonitoring of in vivo genotoxin exposure, in vitro genotoxicity testing and in diverse research fields such as nutrigenomics and pharmacogenomics as well as a predictor of normal tissue and tumor radiation sensitivity and cancer risk. The procedure can take up to 5 days to complete.

引用

页码：1084 / 1104

页数：21

共 108 条

[1] IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans [J].

Albertini, RJ ;

Anderson, D ;

Douglas, GR ;

Hagmar, L ;

Hemminki, K ;

Merlo, F ;

Natarajan, AT ;

Norppa, H ;

Shuker, DEG ;

Tice, R ;

Waters, MD ;

Aitio, A .

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2000, 463 (02) :111-172

[2] A structural analysis of cytoskeleton components during the execution phase of apoptosis [J].

Atencia, R ;

GarciaSanz, M ;

PerezYarza, G ;

Asumendi, A ;

Hilario, E ;

Arechaga, J .

PROTOPLASMA, 1997, 198 (3-4) :163-169

[3] Lymphocytes of BRCA1 and BRCA2 germ-line mutation carriers, with or without breast cancer, are not abnormally sensitive to the chromosome damaging effect of moderate folate deficiency [J].

Beetstra, S ;

Salisbury, C ;

Turner, J ;

Altree, M ;

McKinnon, R ;

Suthers, G ;

Fenech, M .

CARCINOGENESIS, 2006, 27 (03) :517-524

[4]

BEETSTRA S, 2005, MUTAT RES, V78, P317

[5] Telomeres and human disease: Ageing, cancer and beyond [J].

Blasco, MA .

NATURE REVIEWS GENETICS, 2005, 6 (08) :611-622

[6] IMAGE-PROCESSING ALGORITHMS FOR THE AUTOMATED MICRONUCLEUS ASSAY IN BINUCLEATED HUMAN-LYMPHOCYTES [J].

BOCKER, W ;

MULLER, WU ;

STREFFER, C .

CYTOMETRY, 1995, 19 (04) :283-294

[7]

Bonassi S, 2001, ENVIRON MOL MUTAGEN, V37, P31

[8] An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans [J].

Bonassi, Stefano ;

Znaor, Ariana ;

Ceppi, Marcello ;

Lando, Cecilia ;

Chang, Wushou Peter ;

Holland, Nina ;

Kirsch-Volders, Micheline ;

Zeiger, Errol ;

Ban, Sadayuki ;

Barale, Roberto ;

Bigatti, Maria Paola ;

Bolognesi, Claudia ;

Cebulska-Wasilewska, Antonina ;

Fabianova, Eleonora ;

Fucic, Alexandra ;

Hagmar, Lars ;

Joksic, Gordana ;

Martelli, Antonietta ;

Migliore, Lucia ;

Mirkova, Ekaterina ;

Scarfi, Maria Rosaria ;

Zijno, Andrea ;

Norppa, Hannu ;

Fenech, Michael .

CARCINOGENESIS, 2007, 28 (03) :625-631

[9]

Burrill W, 2000, INT J RADIAT BIOL, V76, P375, DOI 10.1080/095530000138718

[10] EFFECTS OF CYTOCHALASINS ON MAMMALIAN CELLS [J].

CARTER, SB .

NATURE, 1967, 213 (5073) :261-&

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