Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium

被引：85

作者：

Chen, Jenny I-C. ^{[1
]}

Hannan, Natalie J. ^{[1
]}

Mak, Yunxian ^{[1
]}

Nicholls, Peter K. ^{[1
]}

Zhang, Jin ^{[1
]}

Rainczuk, Adam ^{[1
]}

Stanton, Peter G. ^{[1
]}

Robertson, David M. ^{[1
]}

Salamonsen, Lois A. ^{[1
]}

Stephens, Andrew N. ^{[1
]}

机构：

[1] Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 04期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

Endometrium; 2D PAGE; two-dimensional differential in-gel electrophoresis (2D DIGE); proteome; proliferative and secretory phases of the menstrual cycle; mass spectrometry; Rho-GDI alpha; CLIC1; PGRMC1; RECEPTOR MEMBRANE COMPONENT-1; EPIDERMAL-GROWTH-FACTOR; PROGESTERONES ANTIAPOPTOTIC ACTION; DISSOCIATION INHIBITOR-ALPHA; ACTIN CYTOSKELETON; BINDING-PROTEIN; GENE-EXPRESSION; MENSTRUAL-CYCLE; CHANNEL; IDENTIFICATION;

D O I：

10.1021/pr801024g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDI alpha, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.

引用

页码：2032 / 2044

页数：13

共 41 条

[1]

Barrett T, 2005, NUCLEIC ACIDS RES, V33, pD562

[2] Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli [J].

Berryman, M ;

Bretscher, A .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (05) :1509-1521

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] Gene expression analysis of endometrium reveals progesterone resistance and candidate susceptibility genes in women with endometriosis [J].

Burney, Richard O. ;

Talbi, Said ;

Hamilton, Amy E. ;

Vo, Kim Chi ;

Nyegaard, Mette ;

Nezhat, Camran R. ;

Lessey, Bruce A. ;

Giudice, Linda C. .

ENDOCRINOLOGY, 2007, 148 (08) :3814-3826

[5] Progesterone receptor membrane component 1: An integrative review [J].

Cahill, Michael A. .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 105 (1-5) :16-36

[6]

COHEN P, 1997, PROTEIN PHOSPHATASE

[7] Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human [J].

Crackower, MA ;

Sinasac, DS ;

Xia, J ;

Motoyama, J ;

Prochazka, M ;

Rommens, JM ;

Scherer, SW ;

Tsui, LC .

GENOMICS, 1999, 55 (03) :257-267

[8] Phosphorylation of RhoGDI by p21-activated kinase 1 [J].

DerMardirossian, CM ;

Bokoch, GM .

METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY, 2006, 406 :80-90

[9] Proteomic analysis of the proliferative and secretory phases of the human endometrium: Protein identification and differential protein expression [J].

DeSouza, L ;

Diehl, G ;

Yang, ECC ;

Guo, JZ ;

Rodrigues, MJ ;

Romaschin, AD ;

Colgan, TJ ;

Siu, KWM .

PROTEOMICS, 2005, 5 (01) :270-281

[10] Expression of the epidermal growth factor system in human endometrium during the menstrual cycle [J].

Ejskjær, K ;

Sorensen, BS ;

Poulsen, SS ;

Mogensen, O ;

Forman, A ;

Nexo, E .

MOLECULAR HUMAN REPRODUCTION, 2005, 11 (08) :543-551

← 1 2 3 4 5 →