Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium

被引：85

作者：

Chen, Jenny I-C. ^{[1
]}

Hannan, Natalie J. ^{[1
]}

Mak, Yunxian ^{[1
]}

Nicholls, Peter K. ^{[1
]}

Zhang, Jin ^{[1
]}

Rainczuk, Adam ^{[1
]}

Stanton, Peter G. ^{[1
]}

Robertson, David M. ^{[1
]}

Salamonsen, Lois A. ^{[1
]}

Stephens, Andrew N. ^{[1
]}

机构：

[1] Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 04期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

Endometrium; 2D PAGE; two-dimensional differential in-gel electrophoresis (2D DIGE); proteome; proliferative and secretory phases of the menstrual cycle; mass spectrometry; Rho-GDI alpha; CLIC1; PGRMC1; RECEPTOR MEMBRANE COMPONENT-1; EPIDERMAL-GROWTH-FACTOR; PROGESTERONES ANTIAPOPTOTIC ACTION; DISSOCIATION INHIBITOR-ALPHA; ACTIN CYTOSKELETON; BINDING-PROTEIN; GENE-EXPRESSION; MENSTRUAL-CYCLE; CHANNEL; IDENTIFICATION;

D O I：

10.1021/pr801024g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDI alpha, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.

引用

页码：2032 / 2044

页数：13

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