Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine

被引：34

作者：

Fernandes, Fabio ^{[1
]}

Loura, Luis M. S.

Fedorov, Alexander

Prieto, Manuel

机构：

[1] Inst Super Tecn, Ctr Quim Fis Mol, P-1096 Lisbon, Portugal

[2] Univ Evora, Ctr Quim, Evora, Portugal

[3] Univ Evora, Dept Quim, Evora, Portugal

来源：

JOURNAL OF LIPID RESEARCH | 2006年 / 47卷 / 07期

关键词：

PIP2; lipid domains; fluorescence; fluorescence resonance energy transfer;

D O I：

10.1194/jlr.M600121-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P-2] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P-2 in plasma membrane domains. Here, we show that PI(4,5)P-2 does not form domains when in a fluid phosphatidylcholine matrix in the pH range of 4.8 - 8.4. This finding is at variance with the spontaneous segregation of PI(4,5)P-2 to domains as a mechanism for the compartmentalization of PI(4,5)P-2 in the plasma membrane. Water/ bilayer partition of PI(4,5)P-2 is also shown to be dependent on the protonation state of the lipid.

引用

页码：1521 / 1525

页数：5

共 24 条

[1] ENERGY-TRANSFER IN SPHERICAL GEOMETRY - APPLICATION TO MICELLES [J].

BERBERANSANTOS, MN ;

PRIETO, MJE .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS II, 1987, 83 :1391-1410

[2] Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis [J].

Botelho, RJ ;

Teruel, M ;

Dierckman, R ;

Anderson, R ;

Wells, A ;

York, JD ;

Meyer, T ;

Grinstein, S .

JOURNAL OF CELL BIOLOGY, 2000, 151 (07) :1353-1367

[3] Dynamics of phosphoinositides in membrane retrieval and insertion [J].

Czech, MP .

ANNUAL REVIEW OF PHYSIOLOGY, 2003, 65 :791-815

[4] TRANSVERSE LOCATION OF THE FLUORESCENT-PROBE 1,6-DIPHENYL-1,3,5-HEXATRIENE IN MODEL LIPID BILAYER-MEMBRANE SYSTEMS BY RESONANCE EXCITATION-ENERGY TRANSFER [J].

DAVENPORT, L ;

DALE, RE ;

BISBY, RH ;

CUNDALL, RB .

BIOCHEMISTRY, 1985, 24 (15) :4097-4108

[5] Cholesterol-dependent partitioning of Ptdlns(4,5)P2 into membrane domains by the N-terminal fragment of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa) [J].

Epand, RM ;

Vuong, P ;

Yip, CM ;

Maekawa, S ;

Epand, RF .

BIOCHEMICAL JOURNAL, 2004, 379 :527-532

[6] PHOSPHOINOSITIDE METABOLISM AND THE MORPHOLOGY OF HUMAN-ERYTHROCYTES [J].

FERRELL, JE ;

HUESTIS, WH .

JOURNAL OF CELL BIOLOGY, 1984, 98 (06) :1992-1998

[7]

Lakowicz JR, 1999, PRINCIPLES FLUORESCE

[8] GAP43, MARCKS, and CAP23 modulate PI(4,5)P2 at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism [J].

Laux, T ;

Fukami, K ;

Thelen, M ;

Golub, T ;

Frey, D ;

Caroni, P .

JOURNAL OF CELL BIOLOGY, 2000, 149 (07) :1455-1471

[9] FLUORESCENCE DEPOLARIZATION STUDIES OF PHASE-TRANSITIONS AND FLUIDITY IN PHOSPHOLIPID BILAYERS .2. 2-COMPONENT PHOSPHATIDYLCHOLINE LIPOSOMES [J].

LENTZ, BR ;

BARENHOLZ, Y ;

THOMPSON, TE .

BIOCHEMISTRY, 1976, 15 (20) :4529-4537

[10] Membrane probe distribution heterogeneity: A resonance energy transfer study [J].

Loura, LMS ;

Fedorov, A ;

Prieto, M .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (29) :6920-6931

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