Cholesterol-dependent partitioning of Ptdlns(4,5)P2 into membrane domains by the N-terminal fragment of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa)

被引：35

作者：

Epand, RM ^{[1
]}

Vuong, P

Yip, CM

Maekawa, S

Epand, RF

机构：

[1] McMaster Univ, Dept Biochem, Hamilton, ON L8N 3Z5, Canada

[2] Univ Toronto, Inst Biomat & Biomed Engn, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3G9, Canada

[3] Kobe Univ, Grad Sch Sci & Technol, Dept Life Sci, Dept Bioinformat,Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

BIOCHEMICAL JOURNAL | 2004年 / 379卷

关键词：

cholesterol; fluorescence; membrane raft; neuronal axonal myristoylated membrane protein of 22 kDa (NAP-22); Ptdlns(4,5)P2; total internal reflectance fluorescence (TIRF) microscopy;

D O I：

10.1042/BJ20040204

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A myristoylated peptide corresponding to the N-terminus of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa) causes the quenching of the fluorescence of BODIPY(R)-TMR-labelled PtdIns(4,5)P-2 in bilayers of 1-palmitoyl-2-oleoyl phosphatidylcholine containing 40 mol % cholesterol and 0.1 mol % BODIPY(R)-Ptdlns(4,5)(2). Both fluorescence spectroscopy and total internal reflectance fluorescence microscopy revealed the cholesterol-dependent nature of PtdIns(4,5)P-2-enriched membrane-domain formation.

引用

页码：527 / 532

页数：6

共 29 条

[1]

Ames B. N., 1966, METHOD ENZYMOL, V8, P115, DOI DOI 10.1016/0076-6879(66)08014-5

[2] Total internal reflection fluorescence microscopy in cell biology [J].

Axelrod, D .

TRAFFIC, 2001, 2 (11) :764-774

[3] Phospholipid/cholesterol model membranes: formation of cholesterol crystallites [J].

Bach, D ;

Wachtel, E .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2003, 1610 (02) :187-197

[4] Production of phosphatidylinositol 3,4,5-trisphosphate and phosphatidic acid in platelet rafts: Evidence for a critical role of cholesterol-enriched domains in human platelet activation [J].

Bodin, S ;

Giuriato, S ;

Ragab, J ;

Humbel, BM ;

Viala, C ;

Vieu, C ;

Chap, H ;

Payrastre, B .

BIOCHEMISTRY, 2001, 40 (50) :15290-15299

[5] Osmotic stress-induced increase of phosphatidylinositol 3,5-bisphosphate requires Vac14p, an activator of the lipid kinase Fab1p [J].

Bonangelino, CJ ;

Nau, JJ ;

Duex, JE ;

Brinkman, M ;

Wurmser, AE ;

Gary, JD ;

Emr, SD ;

Weisman, LS .

JOURNAL OF CELL BIOLOGY, 2002, 156 (06) :1015-1028

[6] Actin cytoskeleton regulation through modulation of PI(4,5)P2 rafts [J].

Caroni, P .

EMBO JOURNAL, 2001, 20 (16) :4332-4336

[7] Binding of basic peptides to membranes produces lateral domains enriched in the acidic lipids phosphatidylserine and phosphatidylinositol 4,5-bisphosphate: An electrostatic model and experimental results [J].

Denisov, G ;

Wanaski, S ;

Luan, P ;

Glaser, M ;

McLaughlin, S .

BIOPHYSICAL JOURNAL, 1998, 74 (02) :731-744

[8] Osmotic stress activates phosphatidylinositol-3,5-bisphosphate synthesis [J].

Dove, SK ;

Cooke, FT ;

Douglas, MR ;

Sayers, LG ;

Parker, PJ ;

Michell, RH .

NATURE, 1997, 390 (6656) :187-192

[9] Specificity of membrane binding of the neuronal protein NAP-22 [J].

Epand, RF ;

Maekawa, S ;

Epand, RM .

JOURNAL OF MEMBRANE BIOLOGY, 2003, 193 (03) :171-176

[10] Shared and unique roles of CAP23 and GAP43 in actin regulation, neurite outgrowth, and anatomical plasticity [J].

Frey, D ;

Laux, T ;

Xu, L ;

Schneider, C ;

Caroni, P .

JOURNAL OF CELL BIOLOGY, 2000, 149 (07) :1443-1453

← 1 2 3 →