The role of pH and membrane porosity in preparative electrophoresis

被引：16

作者：

Corthals, GL

Margolis, J

Williams, KL

Gooley, AA

机构：

[1] MACQUARIE UNIV,SCH BIOL SCI,CTR ANALYT BIOTECHNOL,N RYDE,NSW 2109,AUSTRALIA

[2] GRADIPORE LTD,N RYDE,NSW,AUSTRALIA

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 04期

关键词：

preparative electrophoresis; large-scale electrophoresis; protein purification;

D O I：

10.1002/elps.1150170425

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Gradiflow is a preparative electrophoresis apparatus, allowing fractionation based on a combination of size and charge of proteins in their native (unreduced) form. The preparative fractionation of two proteins of similar size and isoelectric point is demonstrated using the Gradiflow. A separation membrane of appropriate pore size was chosen and then fractionation was ''fine tuned'' by selecting an appropriate buffer pH to accentuate charge differences between the proteins of interest. Complete separation of mg quantities of bovine serum albumin and ovalbumin was achieved within 40 min.*

引用

页码：771 / 775

页数：5

共 9 条

[1]

Hames BD, 1986, GEL ELECTROPHORESIS, P1

[2] SIZE AND CHARGE ISOMER SEPARATION AND ESTIMATION OF MOLECULAR WEIGHTS OF PROTENS BY DISC GEL ELECTROPHORESIS [J].