Hormone autotrophic growth and differentiation identifies mutant lines of Arabidopsis with altered cytokinin and auxin content or signaling

被引:40
作者
Frank, M
Rupp, HM
Prinsen, E
Motyka, V
Van Onckelen, H
Schmülling, T
机构
[1] Univ Tubingen, Ctr Plant Mol Biol, D-72076 Tubingen, Germany
[2] Univ Instelling Antwerp, B-2610 Antwerp, Belgium
[3] Acad Sci Czech Republ, Inst Expt Bot, CZ-16502 Prague 6, Czech Republic
关键词
D O I
10.1104/pp.122.3.721
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We describe mutant tissue lines of Arabidopsis that are able to grow in vitro as callus on hormone-free medium. The 14 lines presented here show different hormone autotrophic differentiation behaviors that can be classified into three categories: (a) forming roots (rooty callus), (b) forming shoots or shoot-like structures (shooty callus), or (c) growing without organ formation (callus). Three fast-growing lines showed altered steady-state mRNA levels of the Cdc2 and CycD3 cell cycle genes. Three of the six rooty callus lines contained about 20- to 30-fold higher levels of auxins than wild-type callus. These and two other lines with normal auxin content showed an increased steady-state level of IAA1 and IAA2 transcripts in the absence of exogenous auxin. Five of the six shooty callus lines had increased steady-state mRNA levels of the CK11 gene and/or of the homeobox genes KNAT1 and STM, suggesting that the phenotype is linked to altered cytokinin signaling. Also, one cytokinin-overproducing line with only 5% of wild-type cytokinin oxidase activity was identified. These results indicate that screening for hormone-autonomous growth identifies mutants with altered hormone content or signaling.
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页码:721 / 729
页数:9
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