Immunohistochemical analysis of oxidative DNA damage in arsenic-related human skin samples from arsenic-contaminated area of China

被引：39

作者：

An, Y

Gao, ZL

Wang, ZW

Yang, SH

Liang, JF

Feng, Y

Kato, K

Nakano, M

Okada, S

Yamanaka, K

机构：

[1] Nihon Univ, Dept Biochem & Toxicol, Coll Pharm, Funabashi, Chiba 2748555, Japan

[2] China Inst Radiat Protect, Taiyuan 030006, Shanxi, Peoples R China

[3] Workers Hosp Yunnan Tin Corp, Gejiu, Peoples R China

[4] Hosp Shanxi Med Univ, Taiyuan 030001, Shanxi, Peoples R China

[5] Natl Chiba Hosp, Chuo Ku, Chiba 2608606, Japan

[6] Univ Shizuoka, Shizuoka 4210103, Japan

来源：

CANCER LETTERS | 2004年 / 214卷 / 01期

关键词：

free radicals; arsenic-related human skin cancers; 8-oxo-2 '-deoxyguanosine (8-oxodG); oxidative stress;

D O I：

10.1016/j.canlet.2004.04.005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The appearance of 8-oxo-2'-deoxyguanosine (8-oxodG) was examined immunohistochemically using an 8-oxodG-monoclonal antibody in 28 cases of arsenic-related human skin tumors and in 20 cases of arsenic-unrelated human skin cancer to determine if the induction of oxidative stress participates in skin tumorigenesis caused by arsenics. The rate of 8-oxodG-positive was significantly higher in arsenic-related human skin cancer (28 of 28, 100%) than in arsenic-unrelated human skin cancer (3 of 20, 15%, P < 0.01 by chi(2) test). Moreover, in all the arsenic-related skin samples, 8-oxodG was detected not only in tumor tissues but also in keratosis and normal tissues. These results suggest that the induction of oxidative stress m ay play an important role in arsenic carcinogenesis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：11 / 18

页数：8

共 35 条

[31] Oral exposure of dimethylarsinic acid, a main metabolite of inorganic arsenics, in mice leads to an increase in 8-oxo-2′-deoxyguanosine level, specifically in the target organs for arsenic carcinogenesis [J].