A cationic water-soluble, poly(p-phenylenevinylene) derivative:: Highly sensitive biosensor for iron-sulfur protein detection a

被引:39
作者
Cheng, Fan
Zhang, Guang-Wei
Lu, Xiao-Mei
Huang, Yan-Qin
Chen, Yi
Zhou, Ying
Fan, Qu-Li
Huang, Wei
机构
[1] Fudan Univ, IAM, Shanghai 200433, Peoples R China
[2] Nanjing Univ, IAM, Nanjing 210093, Peoples R China
关键词
D O I
10.1002/marc.200500867
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
A new water-soluble cationic ammonium-functionalized poly(p-phenylenevinylene) (PPV-NEtMe2+) was successfully synthesized and exhibited high sensitivity (K-sv = 6.9 x 10(7) M-1) on rubredoxin, a type of anionic iron-sulfur (Fe-S) proteins. Further investigation showed that the biosensitivity of the cationic conjugated polymer is strongly dependent on the nature of the buffer solution and the concentration of the conjugated polymer used in the analyses.
引用
收藏
页码:799 / 803
页数:5
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