Characterization of soluble non-covalent complexes between bovine serum albumin and β-1,2,3,4,6-penta-O-galloyl-D-glucopyranose by MALDI-TOF MS

被引：70

作者：

Chen, YM ^{[1
]}

Hagerman, AE ^{[1
]}

机构：

[1] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 12期

关键词：

hydrolyzable tannin; polyphenolic; protein -tannin interaction; pentagalloyl glucose; MALDI-TOF MS;

D O I：

10.1021/jf035536t

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

beta-1,2,3,4,6-Penta-O-galloyl-D-glucopyranose (PGG) and soluble complexes of PGG with bovine serum albumin (BSA) were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PGG was also characterized by electrospray ionization mass spectrometry (ESI-MS). Similar fragmentation patterns of PGG were found in ESI-MS and MALDI-TOF MS. The apparent stoichiometries of non-covalent BSA-PGG complexes were determined by MALDI-TOF MS.

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收藏

页码：4008 / 4011

页数：4

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