Optimisation of a culture medium containing fish silage for L-lysine production by Corynebacterium glutamicum

被引:27
作者
Coello, N
Montiel, E
Concepcion, M
Christen, P
机构
[1] Cent Univ Venezuela, IBE, Lab Proc Biotecnol, Caracas 1041 A, Venezuela
[2] Cent Univ Venezuela, Inst Zool Trop, Caracas 1041, Venezuela
[3] IRD, ESIL, Microbiol Lab, F-13288 Marseille, France
关键词
L-lysine; fish silage; medium optimisation; Corynebacterium glutamicum;
D O I
10.1016/S0960-8524(02)00084-6
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In a first step the effects of 10 components of a culture medium designed for L-lysine production were evaluated with a 2(10-6) factorial design. Among them, glucose, fish silage, and ammonium sulphate showed a significant effect. In a second step, an orthogonal-central composite experimental design and response surface methodology was performed with five from the 10 initial compounds. The determination coefficient (R-2) of the fitted second-order model was 0.990. L-lysine production with the optimised medium increased 2.6 times as compared with the original medium. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:207 / 211
页数:5
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