Optimisation of a culture medium containing fish silage for L-lysine production by Corynebacterium glutamicum

被引：27

作者：

Coello, N

Montiel, E

Concepcion, M

Christen, P

机构：

[1] Cent Univ Venezuela, IBE, Lab Proc Biotecnol, Caracas 1041 A, Venezuela

[2] Cent Univ Venezuela, Inst Zool Trop, Caracas 1041, Venezuela

[3] IRD, ESIL, Microbiol Lab, F-13288 Marseille, France

来源：

BIORESOURCE TECHNOLOGY | 2002年 / 85卷 / 02期

关键词：

L-lysine; fish silage; medium optimisation; Corynebacterium glutamicum;

D O I：

10.1016/S0960-8524(02)00084-6

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In a first step the effects of 10 components of a culture medium designed for L-lysine production were evaluated with a 2(10-6) factorial design. Among them, glucose, fish silage, and ammonium sulphate showed a significant effect. In a second step, an orthogonal-central composite experimental design and response surface methodology was performed with five from the 10 initial compounds. The determination coefficient (R-2) of the fitted second-order model was 0.990. L-lysine production with the optimised medium increased 2.6 times as compared with the original medium. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：207 / 211

页数：5