PNA-Encoded Synthesis (PES) of a 10000-Member Hetero-Glycoconjugate Library and Microarray Analysis of Diverse Lectins

被引：32

作者：

Novoa, Alexandre ^{[1
]}

Machida, Takuya ^{[1
]}

Barluenga, Sofia ^{[1
]}

Imberty, Anne ^{[2
,3
]}

Winssinger, Nicolas ^{[1
]}

机构：

[1] Univ Geneva, Dept Organ Chem, CH-1211 Geneva 4, Switzerland

[2] Univ Grenoble Alpes, CERMAV, F-38000 Grenoble, France

[3] CNRS, F-38000 Grenoble, France

来源：

CHEMBIOCHEM | 2014年 / 15卷 / 14期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

DNA display; glycan array; glycopeptides; microarrays; multivalency; PNA; PEPTIDE NUCLEIC-ACID; PSEUDOMONAS-AERUGINOSA; BINDING LECTIN; SOLID-PHASE; 2-CHLORO-1,3-DIMETHYLIMIDAZOLINIUM CHLORIDE; DIRECTED EVOLUTION; UNPROTECTED SUGARS; TERMINAL ALKYNES; STRUCTURAL BASIS; CONCANAVALIN-A;

D O I：

10.1002/cbic.201402280

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Identification of selective and synthetically tractable ligands to glycan-binding proteins is important in glycoscience. Carbohydrate arrays have had a tremendous impact on profiling glycan-binding proteins and as analytical tools. We report a highly miniaturized synthetic format to access nucleic-acid-encoded hetero-glycoconjugate libraries with an unprecedented diversity in the combinations of glycans, linkers, and capping groups. Novel information about plant and bacterial lectin specificity was obtained by microarray profiling, and we show that a ligand identified on the array can be converted to a high-affinity soluble ligand by straightforward chemistry.

引用

页码：2058 / 2065

页数：8

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