Second-harmonic microscopy of unstained living cardiac myocytes: measurements of sarcomere length with 20-nm accuracy

被引：91

作者：

Boulesteix, T ^{[1
]}

Beaurepaire, E ^{[1
]}

Sauviat, MP ^{[1
]}

Schanne-Klein, MC ^{[1
]}

机构：

[1] Ecole Polytech, CNRS, INSERM, Lab Opt & Biosci, F-91128 Palaiseau, France

来源：

OPTICS LETTERS | 2004年 / 29卷 / 17期

关键词：

D O I：

10.1364/OL.29.002031

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue. (C) 2004 Optical Society of America.

引用

页码：2031 / 2033

页数：3

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