Potentiometric measurement of cell volume changes and intracellular ion concentrations under voltage-clamp conditions in invertebrate nerve cells

被引：7

作者：

Dierkes, PW ^{[1
]}

Coulon, P ^{[1
]}

Neumann, S ^{[1
]}

Schlue, WR ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Neurobiol, D-40225 Dusseldorf, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 373卷 / 08期

关键词：

ion-selective microelectrodes; cell volume; voltage clamp; invertebrate nerve cells;

D O I：

10.1007/s00216-002-1415-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Because changes in cell volume might disturb the normal function of animal cells, most cells are endowed with volume-regulating mechanisms. Experimentally induced changes in cell volume are often paralleled by changes in the membrane potential, which might affect a variety of transport processes across the cell membrane and, in turn, volume regulation [1, 2]. We have shown previously that multi-barrelled ion-selective microelectrodes are useful tools for measurement of cell volume and the intracellular concentrations of ions that might be relevant to volume regulation [1]. To investigate whether voltage-dependent transport processes are involved in cell-volume regulation we combined the potentiometric technique of ion-selective microelectrodes with the voltage-clamp technique. This combination enables simultaneous recording of cell volume, relevant intracellular ion concentrations, and ion currents across the cell membrane at a fixed membrane potential.

引用

页码：762 / 766

页数：5