Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy

被引：59

作者：

Warnasooriya, N. ^{[1
]}

Joud, F. ^{[2
,4
]}

Bun, P. ^{[3
]}

Tessier, G. ^{[1
]}

Coppey-Moisan, M. ^{[3
]}

Desbiolles, P. ^{[2
]}

Atlan, M. ^{[1
]}

Abboud, M. ^{[4
]}

Gross, M. ^{[2
]}

机构：

[1] Ecole Super Phys & Chim Ind Ville Paris, Inst Langevin, CNRS, UMR 7587, F-75231 Paris 05, France

[2] UPMC, CNRS, Ecole Normale Super, Lab Kastler Brossel,UMR 8552, F-75231 Paris 05, France

[3] Univ Paris Diderot, CNRS, Inst Jacques Monod, Programme Biol Cellulaire,UMR 7592, F-75205 Paris 13, France

[4] Univ St Joseph, Fac Sci, Beirut 11072050, Lebanon

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 04期

关键词：

OFF-AXIS; ABSORPTION; SCATTERING;

D O I：

10.1364/OE.18.003264

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environments. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cell environments. (C) 2010 Optical Society of America

引用

页码：3264 / 3273

页数：10

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