Functional analysis of the genes of yeast chromosome V by genetic footprinting

被引：189

作者：

Smith, V

Chou, KN

Lashkari, D

Botstein, D

Brown, PO

机构：

[1] STANFORD UNIV,SCH MED,HOWARD HUGHES MED INST,STANFORD,CA 94305

[2] STANFORD UNIV,SCH MED,DEPT BIOCHEM,STANFORD,CA 94305

[3] STANFORD UNIV,SCH MED,DEPT GENET,STANFORD,CA 94305

来源：

SCIENCE | 1996年 / 274卷 / 5295期

关键词：

D O I：

10.1126/science.274.5295.2069

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.

引用

页码：2069 / 2074

页数：6

共 31 条

[11] MOST OF THE YEAST GENOMIC SEQUENCES ARE NOT ESSENTIAL FOR CELL-GROWTH AND DIVISION
GOEBL, MG
PETES, TD
[J]. CELL, 1986, 46 (07) : 983 - 992
[12] VMA8 ENCODES A 32-KDA V-1 SUBUNIT OF THE SACCHAROMYCES-CEREVISIAE VACUOLAR H+-ATPASE REQUIRED FOR FUNCTION AND ASSEMBLY OF THE ENZYME COMPLEX
GRAHAM, LA
HILL, KJ
STEVENS, TH
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (25) : 15037 - 15044
[13] SEQUENCE OF THE AFG3 GENE ENCODING A NEW MEMBER OF THE FTSH/YME1/TMA SUBFAMILY OF THE AAA-PROTEIN FAMILY
GUELIN, E
REP, M
GRIVELL, LA
[J]. YEAST, 1994, 10 (10) : 1389 - 1394
[14] HARRINGTON A, 1993, MOL MICROBIOL, V9, P545
[15] THE YEAST MOT2 GENE ENCODES A PUTATIVE ZINC-FINGER PROTEIN THAT SERVES AS A GLOBAL NEGATIVE REGULATOR AFFECTING EXPRESSION OF SEVERAL CATEGORIES OF GENES, INCLUDING MATING-PHEROMONE-RESPONSIVE GENES
IRIE, K
YAMAGUCHI, K
KAWASE, K
MATSUMOTO, K
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) : 3150 - 3157
[16] CONTROL OF CELLULAR MORPHOGENESIS BY THE IPL2 BEM2 GTPASE-ACTIVATING PROTEIN - POSSIBLE ROLE OF PROTEIN-PHOSPHORYLATION
KIM, YJ
FRANCISCO, L
CHEN, GC
MARCOTTE, E
CHAN, CSM
[J]. JOURNAL OF CELL BIOLOGY, 1994, 127 (05) : 1381 - 1394
[17] METHIONINE BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE .1. GENETIC-ANALYSIS OF AUXOTROPHIC MUTANTS
MASSELOT, M
ROBICHONSZULMAJSTER, HD
[J]. MOLECULAR & GENERAL GENETICS, 1975, 139 (02): : 121 - 132
[18] SEQUENCE AND CHROMOSOMAL LOCALIZATION OF 2 PET GENES REQUIRED FOR CYTOCHROME-C-OXIDASE ASSEMBLY IN SACCHAROMYCES-CEREVISIAE
MCEWEN, JE
HONG, KH
PARK, S
PRECIADO, GT
[J]. CURRENT GENETICS, 1993, 23 (01) : 9 - 14
[19] MCNEIL JB, 1994, J BIOL CHEM, V269, P9155
[20] 3 REGULATORY SYSTEMS CONTROL PRODUCTION OF GLUTAMINE-SYNTHETASE IN SACCHAROMYCES-CEREVISIAE
MITCHELL, AP
MAGASANIK, B
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (12) : 2767 - 2773

← 1 2 3 4 →