Application of deuterated compounds for investigations of percutaneous absorption of chemical substances

被引:6
作者
Kietzmann, M
Kränke, P
Möder, M
Schrader, S
Wahren, M
机构
[1] Univ Leipzig, Inst Organ Chem, D-04303 Leipzig, Germany
[2] Sch Vet Med, Inst Pharmacol Toxicol & Pharm, Hannover, Germany
[3] UFZ Helmholtz Ctr Environm Res, Ctr Environm Res Leipzig Halle, Dept Analyt Chem, Leipzig, Germany
关键词
bisphenol A; deuterium; dibenzoyl peroxide; isotope dilution analysis; labelled compounds; percutaneous absorption; trace determinations;
D O I
10.1080/10256019908234085
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
The percutaneous absorption of the xenoestrogen 2,2-bis-(4-hydroxyphenyl)-propane (bisphenol A) 1 has been studied and compared with results on dibenzoyl peroxide 2, a component of drug formulations for topical application. Isolated perfused bovine udders from slaughtered cows were employed as models for human skin. The deuterium labelled compounds 1-d(14) and 2-d(10) were applied to enhance the reliability of GC-MS trace determinations by use of reverse isotope dilution analysis. 1-d(14) was found in perfusate and milk equivalent samples obtained between 60 and 300 min after topical application with maximum concentrations between 120 and 180 min. 1-d(14) was enriched in the milk samples by a factor of about 300 compared with the perfusate. The results confirm a possible penetration of 1 from the environment through the skin into the capillary system. 2 studied on the same model system penetrated faster than 1 by a factor of about three.
引用
收藏
页码:127 / 134
页数:8
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