Novel insights into phenotype and mitochondrial proteome of yeast mutants lacking proteins Sco1p or Sco2p

被引：6

作者：

Gamberi, Tania ^{[1
]}

Magherini, Francesca ^{[1
]}

Borro, Marina ^{[2
]}

Gentile, Giovanna ^{[2
]}

Cavalieri, Duccio ^{[3
]}

Marchi, Emmanuela ^{[1
]}

Modesti, Alessandra ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Sci Biochim, I-50134 Florence, Italy

[2] Univ Roma La Sapienza, Dipartimento Sci Biochim, Fac Med 2, Azienda Osped St Andrea, Rome, Italy

[3] Univ Florence, Dipartimento Farmacol, I-50134 Florence, Italy

来源：

MITOCHONDRION | 2009年 / 9卷 / 02期

关键词：

Saccharomyces cerevisiae; Ethanol fermentation; Mitocondrial proteome; Respiratory chain; Respiratory-deficient yeast strain; CALORIE RESTRICTION EXTENDS; CYTOCHROME-C-OXIDASE; SACCHAROMYCES-CEREVISIAE; LIFE-SPAN; CITRATE SYNTHASE; OUTER-MEMBRANE; GENES; TRANSLOCASE; BIOGENESIS; EXPRESSION;

D O I：

10.1016/j.mito.2009.01.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The yeast Saccharomyces cerevisiae is a facultative anaerobe and its mitochondrial morphology is linked to its metabolic activity. The Sco proteins (Sco1p and Sco2p) were characterized as proteins required for copper delivery to cytochrome c oxidase. Our results indicated a higher fermentative capacity of the sco1-Delta mutant in comparison to the control and the sco2-Delta mutant strains. The mitochondrial proteome analysis showed that the sco1-Delta mutant down-regulated components of the respiratory chain, the TCA cycle and transport of metabolites across the mitochondrial membrane. This evidence suggests that the absence of Sco1p causes irreversible damage to the mitochondria. (C) 2009 Elsevier B.V. and Mlitochondria Research Society. All rights reserved.

引用

页码：103 / 114

页数：12

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