Authentication of Panax ginseng from its Adulterants by PCR-RFLP and ARMS

被引：30

作者：

Diao, Ying ^{[1
,2
]}

Lin, Xian-Ming ^{[3
]}

Liao, Chao-Lin ^{[3
]}

Tang, Chun-Zi ^{[3
]}

Chen, Zhong-Jian ^{[4
]}

Hu, Zhong-Li ^{[1
]}

机构：

[1] Wuhan Univ, Coll Life Sci, Key Lab Minist Educ Plant Dev Biol, Wuhan 430072, Peoples R China

[2] Chongqing Univ Arts & Sci, Coll Life Sci & Technol, Chongqing, Peoples R China

[3] Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi, Peoples R China

[4] Wenshan Sanqi Res Inst, Wenshan, Peoples R China

来源：

PLANTA MEDICA | 2009年 / 75卷 / 05期

关键词：

Panax ginseng; Araliaceae; authentication; PCR-RFLP; 5S rDNA NTS; ARMS; MOLECULAR AUTHENTICATION; RAPD ANALYSIS;

D O I：

10.1055/s-0029-1185321

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

As a widely used and expensive herbal medicine, Panax ginseng has many adulterants in the commercial market. PCR-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) based on 5S rDNA sequence analysis were applied to identify two common adulterants of P. ginseng. The sizes of 5S rRNA gene non-transcribed spacers (NTS) sequences in R ginseng and its adulterants were determined, ranging from 143 to 424 bp. The PCR product of P. ginseng only could be digested among the tested specimens because of its specific Spel restriction site found in the 5S rDNA sequence. In addition, P. ginseng was successfully identified from compound medicinal preparations and from the Single-Taste medicines. These results suggest that the methods are able to authenticate P. ginseng.

引用

页码：557 / 560

页数：4

共 12 条

[1]

Molecular phylogenetics of Calamus (Palmae) and related rattan genera based on 5S nrDNA spacer sequence data [J].