High performance liquid chromatographic determination of esculetin and daphnetin in urine and plasma

被引：15

作者：

Egan, DA ^{[1
]}

Duff, C ^{[1
]}

Jordan, L ^{[1
]}

Fitzgerald, R ^{[1
]}

Connolly, S ^{[1
]}

Finn, G ^{[1
]}

机构：

[1] Natl Ctr Sensor Res, Dept Appl Sci, Sch Sci, Inst Technol, Dublin 24, Ireland

来源：

CHROMATOGRAPHIA | 2003年 / 58卷 / 9-10期

关键词：

column liquid chromatography; esculetin and daphnetin; biological samples; internal standard;

D O I：

10.1365/s10337-003-0099-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We have developed and validated a rapid quantitative chromatographic technique and solvent extraction protocol for the quantitation of esculetin and daphnetin in plasma and urine. The linear range was 0.5-100 mug mL(-1). The % recovery for both compounds was found to be in excess of 70%. Intra- and inter-assay precision and accuracy was determined for esculetin and daphnetin following extraction from both plasma and urine, and found to be excellent. It is intended that this method will facilitate the analysis of biological samples obtained from in vivo clinical trials, thereby expediting the pharmacological evaluation of these compounds.

引用

页码：649 / 652

页数：4

共 8 条

[1]

Coumarin glucosides from Cruciata taurica [J].

De Rosa, S ;

Mitova, M ;

Handjieva, N ;

Çalis, I .

PHYTOCHEMISTRY, 2002, 59 (04) :447-450

[2]

In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines [J].

Finn, GJ ;

Kenealy, E ;

Creaven, BS ;

Egan, DA .

CANCER LETTERS, 2002, 183 (01) :61-68

[3]

LI ZG, 1993, ZHONGGUO ZHONG YUA Z, V509, P458

[4]

Analysis of flavonoids and coumarins in Ixeris laevigata var. Oldhami by HPLC [J].

Lu, KL ;

Ku, YR ;

Wen, KC ;

Ho, LK ;

Chang, YS .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (16) :2573-2583

[5]

SIMULTANEOUS DETERMINATION OF AROMATIC-ALDEHYDES AND COUMARINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - APPLICATION TO WINES AND BRANDIES STORED IN OAK BARRELS [J].

SALAGOITYAUGUSTE, MH ;

TRICARD, C ;

SUDRAUD, P .

JOURNAL OF CHROMATOGRAPHY, 1987, 392 :379-387

[6]

Identification and characterisation of the Chinese herb Langdu by LC-MS/MS analysis [J].

Su, XL ;

Lin, RC ;

Wong, SK ;

Tsui, SK ;

Kwan, SY .

PHYTOCHEMICAL ANALYSIS, 2003, 14 (01) :40-47

[7]

Simultaneous blood and biliary sampling of esculetin by microdialysis in the rat [J].

Tsai, TH ;

Huang, CT ;

Shum, AYC ;

Chen, CF .

LIFE SCIENCES, 1999, 65 (16) :1647-1655

[8]

Studies on the hepatocyte protective activity and the structure-activity relationships of quinic acid and caffeic acid derivatives from the flower buds of Lonicera bournei [J].

Xiang, T ;

Xiong, QB ;

Ketut, AI ;

Tezuka, Y ;

Nagaoka, T ;

Wu, LJ ;

Kadota, S .

PLANTA MEDICA, 2001, 67 (04) :322-325

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