Mass spectrometric identification of serine hydrolase OVCA2 in the medulloblastoma cell line DAOY

被引：10

作者：

Azizi, Amedeo A.

Gelpi, Ellen

Yang, Jae-Won

Rupp, Bernhard

Godwin, Andrew K.

Slater, Carolyn

Slavc, Irene

Lubec, Gert

机构：

[1] Med Univ Vienna, Dept Pediat, A-1090 Vienna, Austria

[2] Med Univ Vienna, Clin Inst Neurol, A-1090 Vienna, Austria

[3] Lawrence Livermore Natl Lab, Biosci Div L448, Livermore, CA USA

[4] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA

来源：

CANCER LETTERS | 2006年 / 241卷 / 02期

基金：

美国国家卫生研究院;

关键词：

OVCA2; medulloblastoma; two-dimensional gel electrophoresis; mass-spectrometry; immunohistochemistry;

D O I：

10.1016/j.canlet.2005.10.023

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

OVCA2 is a putative serine-hydrolase. Performing protein profiling in human tumour cell lines, OVCA2 was detected in DAOY medulloblastoma cells as a high abundance protein. The protein was unambiguously identified by 2D gel-electrophoresis and MALDI-MS and MS/MS, its presence was confirmed by western blotting. Immunohistochemistry revealed expression in medulloblastoma and predominantly in oligodendrocytes. Computational approaches predicted functional motifs and domains, interaction with apoptosis-related protein BAG and 3D structure. In addition to the presence of OVCA2 in medulloblastoma, it was furthermore detectable in three out of 10 human tumour cell-lines as a high abundance protein probably suggesting a role in the tumour biology. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：235 / 249

页数：15

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