Semisynthesis of a Homogeneous Glycoprotein Enzyme: Ribonuclease C: Part 2

被引：90

作者：

Piontek, Christian ^{[1
]}

Silva, Daniel Varon ^{[1
]}

Heinlein, Christian ^{[1
]}

Poehner, Claudia ^{[1
]}

Mezzato, Stefano ^{[1
]}

Ring, Petra ^{[1
]}

Martin, Andreas ^{[1
]}

Schmid, Franz Xaver ^{[1
]}

Unverzagt, Carlo ^{[1
]}

机构：

[1] Univ Bayreuth, D-95440 Bayreuth, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 11期

关键词：

glycoproteins; glycosylation; native chemical ligation; protecting groups; ribonucleases; TOTAL CHEMICAL-SYNTHESIS; SOLID-PHASE SYNTHESIS; CHEMOENZYMATIC SYNTHESIS; GLYCOPEPTIDES; PEPTIDE; GLYCOSYLATION; PROTEINS; TOOLS;

D O I：

10.1002/anie.200804735

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1941 / 1945

页数：5

共 43 条

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[2]

[Anonymous], 2005, ANGEW CHEM, V117, P1677

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