Hemicellulolytic activity of Fusarium oxysporum grown on sugar beet pulp.: Production of extracellular arabinanase

被引:31
作者
Cheilas, T [1 ]
Stoupis, T [1 ]
Christakopoulos, P [1 ]
Katapodis, P [1 ]
Mamma, D [1 ]
Hatzinikolaou, DG [1 ]
Kekos, D [1 ]
Macris, BJ [1 ]
机构
[1] Natl Tech Univ Athens, Dept Chem Engn, Biotechnol Lab, Athens, Greece
关键词
arabinanase production; Fusarium oxysporum; sugar-beet pulp;
D O I
10.1016/S0032-9592(99)00103-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fusarium oxysporum F3 exhibited hemicellulolytic enzymic activity when grown on sugar beet pulp, a by-product of the sugar industry. The growth medium was specifically optimised for enhanced production of extracellular arabinanase. The optimum medium contained sugar beet pulp (4%, w/v) and corn steep liquor (6%, v/v) as carbon and nitrogen sources, respectively. Arabinanase activity as high as 0.25 U/ml of culture was obtained, which compared favourably to those reported for other microorganisms. Optimal arabinanase activity was observed at pH 6-7 and 50 degrees C. Investigation of the degradation of the main components of sugar beet pulp showed that arabinose containing polysaccharides and pectin were first degraded, followed by the glucose-containing polysaccharides. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:557 / 561
页数:5
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