Hemicellulolytic activity of Fusarium oxysporum grown on sugar beet pulp.: Production of extracellular arabinanase

被引：31

作者：

Cheilas, T ^{[1
]}

Stoupis, T ^{[1
]}

Christakopoulos, P ^{[1
]}

Katapodis, P ^{[1
]}

Mamma, D ^{[1
]}

Hatzinikolaou, DG ^{[1
]}

Kekos, D ^{[1
]}

Macris, BJ ^{[1
]}

机构：

[1] Natl Tech Univ Athens, Dept Chem Engn, Biotechnol Lab, Athens, Greece

来源：

PROCESS BIOCHEMISTRY | 2000年 / 35卷 / 06期

关键词：

arabinanase production; Fusarium oxysporum; sugar-beet pulp;

D O I：

10.1016/S0032-9592(99)00103-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fusarium oxysporum F3 exhibited hemicellulolytic enzymic activity when grown on sugar beet pulp, a by-product of the sugar industry. The growth medium was specifically optimised for enhanced production of extracellular arabinanase. The optimum medium contained sugar beet pulp (4%, w/v) and corn steep liquor (6%, v/v) as carbon and nitrogen sources, respectively. Arabinanase activity as high as 0.25 U/ml of culture was obtained, which compared favourably to those reported for other microorganisms. Optimal arabinanase activity was observed at pH 6-7 and 50 degrees C. Investigation of the degradation of the main components of sugar beet pulp showed that arabinose containing polysaccharides and pectin were first degraded, followed by the glucose-containing polysaccharides. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：557 / 561

页数：5