The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1

被引：215

作者：

Zhuang, Ming ^{[1
,2
]}

Gao, Wen ^{[1
]}

Xu, Jing ^{[1
]}

Wang, Ping ^{[1
]}

Shu, Yongqian ^{[1
]}

机构：

[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Oncol, Nanjing 210029, Jiangsu, Peoples R China

[2] First Peoples Hosp Lianyungang, Dept Oncol, Lianyungang 210029, Jiangsu, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 448卷 / 03期

基金：

中国国家自然科学基金;

关键词：

H19; miR-675; RUNX1; Gastric cancer; Cell proliferation; DIFFERENTIAL EXPRESSION; H19; LINCRNA;

D O I：

10.1016/j.bbrc.2013.12.126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The IncRNA H19 has been recently shown to be upregulated and play important roles in gastric cancer tumorigenesis. However, the precise molecular mechanism of H19 and its mature product miR-675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that miR-675 was positively expressed with H19 and was a pivotal mediator in H19-induced gastric cancer cell growth promotion. Subsequently, the tumor suppressor Runt Domain Transcription Factor1 (RUNX1) was confirmed to be a direct target of miR-675 using a luciferase reporter assay and Western blotting analyses. A series of rescue assays indicated that RUNX1 mediated H19/miR-67-induced gastric cancer cell phenotypic changes. Moreover, the inverse relationship between the expression of RUNX1 and H19/miR-675 was also revealed in gastric cancer tissues and gastric cancer cell lines. Taken together, our study demonstrated that the novel pathway H19/miR-675/RUNX1 regulates gastric cancer development and may serve as a potential target for gastric cancer therapy. (C) 2014 Published by Elsevier Inc.

引用

页码：315 / 322

页数：8

共 36 条

[1]

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[2]

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