Examination of IGF2 and H19 loss of imprinting in bladder cancer

被引:114
作者
Byun, Hyang-Min
Wong, Hui-Lee
Birnstein, Elliott Aaron
Wolff, Erika M.
Liang, Gangning
Yang, Allen S.
机构
[1] Univ So Calif, Kenneth Norris Jr Comprehens Canc Ctr, Div Hematol, Los Angeles, CA 90033 USA
[2] Univ So Calif, Kenneth Norris Jr Comprehens Canc Ctr, Dept Biochem, Los Angeles, CA 90033 USA
[3] NCI, Viral Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,Dept Hlth & Human Serv, Bethesda, MD 20892 USA
关键词
D O I
10.1158/0008-5472.CAN-07-0329
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Loss of imprinting (1,01) is a common epigenetic event in cancer and may serve as an early biomarker in some cancers. To obtain a better understanding of 1,01, we studied 41 bladder tumors and their adjacent normal bladder mucosa. We found 2/9 (22.2%) cases that displayed LOI of IGF2 and 2/16 (12.5%) that had LOI of H19, as determined by the evaluation of mRNA for biallelic expression. In addition, we examined allele-specific methylation of the differentially methylated regions (DMR) of IGF2 and H19 using a new allele-specific pyrosequencing assay. We found that DNA methylation changes were a common finding (21/30, 70%) in the DMR regions, but could not clearly link DNA methylation changes with LOI as measured by biallelic expression. LOI and allele-specific DNA methylation changes are present in bladder cancer; however, a better understanding of the biology of LOI and its relationship to DNA methylation changes is needed before its use as an epigenetic biomarker.
引用
收藏
页码:10753 / 10758
页数:6
相关论文
共 32 条
[1]   Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene [J].
Bell, AC ;
Felsenfeld, G .
NATURE, 2000, 405 (6785) :482-485
[2]  
CLARK SJ, 1994, NUCLEIC ACIDS RES, V22, P2990, DOI 10.1093/nar/22.15.2990
[3]   Loss of imprinting in normal tissue of colorectal cancer patients with microsatellite instability [J].
Cui, HM ;
Horon, IL ;
Ohlsson, R ;
Hamilton, SR ;
Feinberg, AP .
NATURE MEDICINE, 1998, 4 (11) :1276-1280
[4]   Loss of IGF2 imprinting:: A potential marker of colorectal cancer risk [J].
Cui, HM ;
Cruz-Correa, M ;
Giardiello, FM ;
Hutcheon, DF ;
Kafonek, DR ;
Brandenburg, S ;
Wu, YQ ;
He, XB ;
Powe, NR ;
Feinberg, AP .
SCIENCE, 2003, 299 (5613) :1753-1755
[5]  
Cui HM, 2001, CANCER RES, V61, P4947
[6]  
Cui HM, 2002, CANCER RES, V62, P6442
[7]   Prevalence of aberrant methylation of p14ARF over p16INK4a in some human primary tumors [J].
Dominguez, G ;
Silva, J ;
Garcia, JM ;
Silva, JM ;
Rodriguez, R ;
Muñoz, C ;
Chacón, I ;
Sanchez, R ;
Carballido, J ;
Colás, A ;
España, P ;
Bonilla, F .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2003, 530 (1-2) :9-17
[8]   THE EXPRESSION OF THE IMPRINTED H19 AND IGF-2 GENES IN HUMAN BLADDER-CARCINOMA [J].
ELKIN, M ;
SHEVELEV, A ;
SCHULZE, E ;
TYKOCINSKY, M ;
COOPER, M ;
ARIEL, I ;
PODE, D ;
KOPF, E ;
DEGROOT, N ;
HOCHBERG, A .
FEBS LETTERS, 1995, 374 (01) :57-61
[9]   Genetic imprinting: Silencing elements have their say [J].
Ferguson-Smith, AC .
CURRENT BIOLOGY, 2000, 10 (23) :R872-R875
[10]   A potential imprint control element -: identification of a conserved 42 bp sequence upstream of H19 [J].
Frevel, MAE ;
Hornberg, JJ ;
Reeve, AE .
TRENDS IN GENETICS, 1999, 15 (06) :216-218