Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF-/- mice

被引：43

作者：

Bel, Shai ^{[1
]}

Elkis, Yoav ^{[1
]}

Elifantz, Hila ^{[1
]}

Koren, Omry ^{[3
]}

Ben-Hamo, Rotem ^{[1
]}

Lerer-Goldshtein, Tal ^{[1
]}

Rahimi, Roni ^{[1
]}

Ben Horin, Shomron ^{[4
]}

Nyska, Abraham ^{[5
]}

Shpungin, Sally ^{[1
]}

Nir, Uri ^{[1
,2
]}

机构：

[1] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-5290002 Ramat Gan, Israel

[2] Bar Ilan Univ, Quaresa Inst Canc Res, IL-5290002 Ramat Gan, Israel

[3] Bar Ilan Univ, Fac Med, IL-1311502 Safed, Israel

[4] Chaim Sheba Med Ctr, Dept Gastroenterol, IL-52621 Tel Hashomer, Israel

[5] Tel Aviv Univ, Sackler Sch Med, IL-36576 Tel Aviv, Israel

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 13期

关键词：

co-housing; inflammatory bowel disease; mucus granule; ENTERIC PATHOGENS; EPITHELIAL-CELLS; GUT MICROBIOTA; MUC2; MUCIN; COLONIZATION; TMF/ARA160; DYSBIOSIS; EXERTS; DOMAIN; MODEL;

D O I：

10.1073/pnas.1319114111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tata Element Modulatory Factor (TMF/ARA160) is a multifunctional Golgi-associated protein, which accumulates in colonic enterocytes and goblet cells. Mice lacking TMF/ARA160 (TMF-/-) produce thick and uniform colonic mucus that resists adherent bacterial colonization and diminishes susceptibility of these mice to induced acute colitis, through a mechanism that is not fully understood. Here, we show that mucus secretion by goblet cells is altered in the colon of TMF-/- mice, resulting in the formation of a highly oligomerized colonic gel-forming mucin, MUC2. Microbiome analysis revealed a shift in the microbiota of TMF-/- mice leading to predominance of the Firmicutes phylum and a significantly higher abundance of probiotic beneficial bacterial species. Notably, this trait was transmissible, and when cohoused with wild-type animals, TMF-/- mice influenced the microbiota and diminished the susceptibility of wildtype mice to chemically induced dextran sulfate sodium colitis. Thus, altered mucus secretion in TMF-/- mouse colons is accompanied by a reprogrammed intestinal microbiota, leading to a transmissible reduced sensitivity to induced colitis.

引用

页码：4964 / 4969

页数：6

共 33 条

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